help: pMOG

Francisco Villamon fvillamon at hotmail.com
Sat Feb 6 13:30:11 EST 1999


Hi

I am working with pMOG and I have problem trying to insert a 2 kb 
fragment. When I digest  using a single cutting point enzyme (in not 
transformed pMOG) to verify the plasmid size and indentify the 
transformed colonies appear a smire in agarose gel. A lot of people told 
me my miniprep protocol is not good due a missing band corresponding  to 
supercoiled form.
Does anybody have experience with pMOG?
Does anyone know a reliable protocol-made at home- for plasmid 
extraction? 
All help will be welcome
Thanks in advanced

Francisco

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