Restriction enzyme cutting condition

Andrew Leung leungkc at glink.net.hk
Mon Feb 8 10:19:36 EST 1999


Hi all,
        I have frequently got incomplete cut in my restriction enzyme
cutting reaction. So I wonder if my reaction got any problem. Generally, I
cut 1ug plasmid with 5U of enzyme in a 20ul reaction and incubate overnight.
However, it is always only half the plasmid is cut upon examine in gel. Is
there any advice? Is enzyme too much or volumn of reaction needs scaled?

Andrew





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