zxn at PENTIUM.WHIOV.AC.CN
Mon Feb 8 20:51:13 EST 1999
I have two questions to ask you:
1.I have done IS-PCR for several times ,but the results were not interesting.
After added PCR reaction system to the surface of the slide, I covered the
liquid with coverslip,then I sealed the brim of the coverslip with nail
oil as discribled
in RED BOOK,then put them on a PE thermo cycler,but after heated to 94
the seal of nail oil was often broken out by the force of liquid
expand,then the liquid
would ran away quikly from the leakage ,finally ,I got nothing after
I don't know how to overcome this problem and seal better just by nail
oil,and are there have other quick ,easy , practicable,and cheap methods
for this seal procedure in IS-PCR?
2.Are the PCR components easy attach to the the glass surface during PCR
and how to overcome them ?
a.Protein(Taq polymerase )
Tao sheng ce
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