Dithiothreitol (DTT) cf 2-mercaptoethanol (2ME)

[kevin gilbride]

In article <Pine.OSF.3.95.990126164423.20371C-100000 at dingo.cc.uq.edu.au>,
"Paul R. Rohde" <mdprohde at mailbox.uq.edu.au> wrote:

> Just a general question,
> (but maybe perhaps biased towards RNA extractions).
> 
> Whats the difference between DTT and 2ME?  What does one chemical do 
> that the other doesn't, and when should each be used? 
> 
> How many moles of DTT equals moles of 2ME (in effectiveness)?
> 
> Is 2ME more potent as it stinks more and more nauseating?
> They are both in the lab, should know more about them.
> 
> 
> 
> _______________________________________________________
> Paul R. Rohde   http://www.uq.edu.au/~mdprohde/               *    *
> Department of Surgery, University of Queensland
> Royal Brisbane Hospital, Queensland, 4029, Australia             . *
> FAX: -Australia- +61 7 3365 5559  TEL: +61 7 3362 0336       *
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Hello,
I have use 2ME to break disulfide bonds in proteins (denaturing) for
electrophoresis. How is it used in RNA extraction.

KJ Gilbride
gilbride at bu.edu