expression of zinc-finger containing proteins in E.coli

Svend Kjaer Svend at CAJAL.MBB.KI.SE
Wed Feb 10 11:39:57 EST 1999

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Hi there,

We are about to embark on expression and purification of a zinc-finger
containing protein in E. coli. Are there any tricks to achieve soluble
protein, i.e. have you seen whether addition of zinc to the bugs during
expression of the protein makes any differences ??

Any input appreciated,

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