expression of zinc-finger containing proteins in E.coli

Svend Kjaer Svend at CAJAL.MBB.KI.SE
Wed Feb 10 11:39:57 EST 1999


This is a multi-part message in MIME format.
--------------38B0C3DFFC9BB3CDEA780DF4
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit

Hi there,

We are about to embark on expression and purification of a zinc-finger
containing protein in E. coli. Are there any tricks to achieve soluble
protein, i.e. have you seen whether addition of zinc to the bugs during
expression of the protein makes any differences ??

Any input appreciated,

Svend
--------------38B0C3DFFC9BB3CDEA780DF4
Content-Type: text/x-vcard; charset=us-ascii; name="vcard.vcf"
Content-Transfer-Encoding: 7bit
Content-Description: Card for Svend Kjaer
Content-Disposition: attachment; filename="vcard.vcf"

begin:          vcard
fn:             Svend Kjaer
n:              Kjaer;Svend
org:            Division of Molecular Neurobiology; Department of Neuroscience; Karolinska Institute
adr:            ;;Doktorsringen 12B, Plan 3;Stockholm;Sweden;17177;
email;internet: Svend at cajal.mbb.ki.se
title:          Postdoctoral Fellow
tel;work:       0046-8-7287666
tel;fax:        0046-8-339548
tel;home:       0046-8-6406692
x-mozilla-cpt:  ;0
x-mozilla-html: FALSE
end:            vcard


--------------38B0C3DFFC9BB3CDEA780DF4--





More information about the Methods mailing list