Recycling electroporation cuvettes

MOUNIR IZALLALEN aah313 at agora.ulaval.ca
Fri Feb 12 13:35:47 EST 1999


Hi,

After theelectroporation don't let it stand too long, the culture tend to
harden.
I rinse them with tap water, and I add a few drops of ELIMINASE
(Fisher:04-355-31), then thouroughly rinse them with distilled water.Then
I add Ethanol 90% and cap them for a couple of days. Then under the steril
hood I change the 90% EtOH to 70% and let it dry with UV on.
I recycle them at least 3 or 4 times this way.

Hope it helps

Mounir
==============================================
Mounir Izallalen
Université Laval
Recherche en Sciences de la vie et de la Sante
Pavillon C. E. Marchand
Ste-Foy, G1K 7P4
Québec.
Canada
Tel. (418) 656-2131 ext# 6785
Fax. (418) 656-7176
===============================================




More information about the Methods mailing list