isolation of mitochondrial fractions

[Andreas Becker]

Dear Sir


I am working on  neuronal apoptosis and my interest is to study the
Cyt c release from mitochondria to cytosol .Please provide me the
suitable protocol  for the Cyt c estimationin mitochondrial and
cytosolic fractions by western blotting

I could also appreciate further suggestions in this regard.


Thanking you

Pratima Thakur
Institute for Pharmacology and Toxikology 
Marburg 
Germany  

my e-mail address--pratimathakur at hotmail.com