PCR Nightmare

Leslie Hutton lahutton at utmb.edu
Fri Feb 12 11:38:52 EST 1999


I have been haveing problems with background in my RT-PCR reactions. I
do not see bands when the RT or RNA is left out of my reaction. However,
if I add RNA which has not been reverse transcribed to the reaction I
see bands. DNase I digestion of the sample does not eliminate this
problem nor does it occur with all of my primer sets. Has this ever
happened to anyone else? What is the cause and how do I eliminate it?
DNase I digestion does not help. \

thank you for your reply




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