pET fusion vector

Marcus Wernitz wernitz at landw.uni-halle.de
Fri Feb 12 10:19:31 EST 1999


Hi Han Bok Kim,

Maybe you can get it from the NIG. Send a search result for "pET":
go to CVectorDB Home or search again 



             Name: pET control A
         Replicon: pMB1
             Size: 
          Purpose: Control
Selectable Marker: amp
      Other Genes: lacZ
         Promoter: PT7
     Cloning Site: 
        Reference: 
           Source: Novagen
              Map: No Map data



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             Name: pET control B
         Replicon: pMB1
             Size: 
          Purpose: Control
Selectable Marker: amp
      Other Genes: lacZ
         Promoter: PT7 
     Cloning Site: 
        Reference: 
           Source: Novagen
              Map: No Map data



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             Name: pET-3af1
         Replicon: pMB1/f1
             Size: 5.165
          Purpose: Expression
Selectable Marker: amp
      Other Genes: 
         Promoter: PT7
     Cloning Site: BamHI HindIII NdeI EcoRV
        Reference: Gene 118, 103-107 (1992j
           Source: J. P. Richardson
              Map: pET-3af1.gif



------------------------------------------------------------

             Name: pET-His
         Replicon: pMB1
             Size: 4.5
          Purpose: Fusion, Promoter Cloning
Selectable Marker: amp
      Other Genes: his tag, T7gene10 terminator
         Promoter: PT7gene10
     Cloning Site: BamHI XhoI NcoI
        Reference: Gene 139, 73-75 (1994)
           Source: B. Chen
              Map: pET-His.gif



------------------------------------------------------------

             Name: pET-HisK
         Replicon: pMB1
             Size: 4.5
          Purpose: Fusion, Promoter Cloning
Selectable Marker: amp
      Other Genes: his tag, HMK site, T7gene10terminator
         Promoter: PT7gene10
     Cloning Site: BamHI XhoI NcoI
        Reference: Gene 139, 73-75 (1994)
           Source: B. Chen
              Map: pET-HisK.gif



------------------------------------------------------------

             Name: pET15b
         Replicon: pMB1
             Size: 5.708
          Purpose: Fusion/Expression
Selectable Marker: amp
      Other Genes: lacI, his tag, thrombin site, T7 terminator
         Promoter: PT7, Plac
     Cloning Site: BamHI XhoI NdeI
        Reference: 
           Source: Novagen
              Map: pET15b.gif



------------------------------------------------------------

             Name: pET16b
         Replicon: pMB1
             Size: 5.711
          Purpose: Fusion/Expression
Selectable Marker: amp
      Other Genes: lacI, his tag, Xa site, T7 terminator
         Promoter: PT7, Plac
     Cloning Site: BamHI XhoI NdeI
        Reference: 
           Source: Novagen
              Map: pET16b.gif



------------------------------------------------------------

             Name: pET19b
         Replicon: pMB1
             Size: 5.717
          Purpose: Fusion/Expression
Selectable Marker: amp
      Other Genes: lacI, Enterokinase site, T7 terminator
         Promoter: PT7, Plac
     Cloning Site: BamHI XhoI NdeI
        Reference: 
           Source: Novagen
              Map: pET19b.gif



------------------------------------------------------------

             Name: pET20b(+)
         Replicon: pMB1/f1
             Size: 3.716
          Purpose: Expression
Selectable Marker: amp
      Other Genes: 
         Promoter: PT7 
     Cloning Site: BamHI EcoRI HindIII SalI SmaI XhoI NdeI NcoI EcoRV SacI NotI
EagI
        Reference: 
           Source: Novagen
              Map: pET20b(+).gif



------------------------------------------------------------

             Name: pET21(+)
         Replicon: pMB1/f1
             Size: 5.369
          Purpose: Expression
Selectable Marker: amp
      Other Genes: lacI
         Promoter: PT7 
     Cloning Site: BamHI EcoRI HindIII SalI XhoI SacI EagI NotI
        Reference: 
           Source: Novagen
              Map: pET21(+).gif



------------------------------------------------------------

             Name: pET24(+)
         Replicon: pMB1/f1
             Size: 5.236
          Purpose: Expression
Selectable Marker: kan
      Other Genes: lacI
         Promoter: PT7 
     Cloning Site: BamHI EcoRI HindIII SalI XhoI SacI EagI NotI
        Reference: 
           Source: Novagen
              Map: pET24(+).gif



------------------------------------------------------------

             Name: pETGEXCT
         Replicon: pMB1
             Size: 5
          Purpose: Fusion
Selectable Marker: amp
      Other Genes: GST, thrombin site
         Promoter: PT7
     Cloning Site: BamHI NcoI SacI
        Reference: Gene 138, 105-108 (1994)
           Source: A. D. Sharrocks
              Map: pETGEXCT.gif



------------------------------------------------------------


and the link where and how you can get plasmids:
http://shigen.lab.nig.ac.jp/cvector.html
Hope it helps,
marcus
funginetic at geocities.com


Han Bok Kim schrieb:
> 
> I knew that there is an expression system of pET fusion. It uses phageT7
> expression system and fusion signal for Ni for easy purification. I forgot
> the company's name selling the product. I would apprediate it.
> 
> Han



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