Proteinase K to get rid of RNase?

Gen Yan Yang gyang at MED.UNC.EDU
Fri Feb 12 20:44:27 EST 1999


Does anyone know if it is possible to digest DNA primer for RT using
proteinase K in order to get rid of RNase? Proteinase K can then be
taken away by extraction using penol/chloroform?  I have checked the
proteinase K is RNase free by incubation RNA with it for 2 hours @37
c    Thanks for any suggestion!

Yang
UNC-CH, USA



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