Microscope Design Concept which eliminates the need for staining cell tissues.

The Flavored Coffee Guy elgersmad at email.msn.com
Sun Feb 14 03:30:34 EST 1999


http://pc65.frontier.osrhe.edu/hs/science/emspec.gif


    Looking at the radio spectrum in order to define which compounds, and
elements are present have frequently been used in astronomy.  But, in the
field of microscopy this form of information gathering isn't utilized.  In
this post are links that consist of the parts of highest significance
required to build a microscope that doesn't require stains, or staining
procedures to view cell tissues.  A tunable laser is used as a light source
in order to align the spectral lines generated in spectroscopy associated to
a sample being viewed.  Minimum amounts of light enable the reflectivity, or
absorption lines found to either be reflected or absorbed within in a
minimum number of atoms, or molecules.  Therefore, the image multiplier tube
is used to amplify what little light is used to unveil the internal, or
external structures of the cell being examined.  Basing the lasers output
frequency  to be an issue resolved by computer controls, stepping the laser
through it's output frequency range.   Retrieving a series of images
produced on a one image, per step in frequency would enable software to
retrieve, and compile an image based on the absorbed, or reflected
wavelengths of light.  Accumulating this information using software to
generate a multiple of grayscale images which would be placed in the visible
range of colors generated by a computer by spacing the gray images in a
false color spectrum map, then letting the computer compress the scale into
the visible range for viewing.

    Based on the objective of viewing the translucent cells, such as neural
tissues, and doing so without damaging them.  The light exiting the source
is polarized in order to determine the polarize state of molecules which are
being viewed.  If this is included in the frequency/image stepping sequence,
several images will need to be take based on the degree of rotation of the
interference lenses rotation.  This lens is a secondary rotatable polarized
lens that is used for interference to detect the degree at which polarized
light rotates passing through any given molecule.

    Further stepping modifications include detailing focal lengths, and
calibrating a robotic drive assembly to generate a 3 dimensional model of a
cell in a computer based on objective, and focal length.  This would amount
to either an auto focus system, or focusing on the highest point of a cell,
and then the slide.  From there the computer would asses the cell in steps
in the first order.

    If the microscopes software follows through this full featured design
sequence properly the order of events are as follows.

1. Objective start step, and finish step pinion settings.
2. Step 1 of the tunable laser frequency, either the highest, or lowest.
3. Polarized Rotation stepping and images are accumulated.
4. Next Objective step.
5. Goto step 3 until Objective step = last step.
6. Next laser output frequency.
7. Goto 3 until laser output frequency = last frequency.

A binary search would be used to find the image files which contained the
highest contrast, and mark, or rename each file.  Then use the compressed
light range to generate a complete image out of overlaying the images
produced in the grayscale format.  I have leave you with the three
dimensional stuff, because that's a trigonometric function of boxes in a
box.


Selected tunable laser Diode sites

http://www.maycomm.com/
http://aesd.larc.nasa.gov/C/CF/CF12f.html

Robotics positioner assemblies.
http://mwrn.com/product/light_microscopy/accessories.htm


Instrument grade low light Imaging tube spec sheet.
http://www.burle.com/cgi-bin/byteserver.pl/pdf/4804h.pdf


The Flavored Coffee Guy.










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