JOHN-F.MACKAY at roche.com
Mon Feb 15 00:06:31 EST 1999
how large are these products that are showing bands? Taq does have
some RT activity. If the thermalcycler ramps slowly, the product is
small aand there is a lot of RNA added then this may be the cause.
Another check would be to add some DNase-free RNase to the reaction
which should result in a blank reaction. Bands would mean DNA
contamination as opposed to Taq RT activity.......
Roche Diagnostics NZ
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