PCR Nightmare

John-F Mackay JOHN-F.MACKAY at roche.com
Mon Feb 15 00:06:31 EST 1999


     
     Leslie,
     
     how large are these products that are showing bands? Taq does have 
     some RT activity. If the thermalcycler ramps slowly, the product is 
     small aand there is a lot of RNA added then this may be the cause.
     Another check would be to add some DNase-free RNase to the reaction 
     which should result in a blank reaction. Bands would mean DNA 
     contamination as opposed to Taq RT activity.......
     
     Kind regards,
     
     John
     Roche Diagnostics NZ




More information about the Methods mailing list