Finding restrictase by recognition sequence

alex dobrovic adobrovic at medicine.adelaide.edu.au
Mon Feb 15 01:30:06 EST 1999


>In article <36C0AB97.E1DCDDD7 at scientist.com>, Max Myakishev
><max at scientist.com> writes
>>For example,
>>acgtgt (G/A)aatatcga
>>I would like to design a PCR/RFLP assay for genotyping this SNP.
>>How do I find a restriction enzyme that recognizes the difference in
>>this sequence?

Design a forward primer with a 3' mismatch in the penultimate base:
NNNNNNNNNNNNNNacgtTt.
This then creates a Dra1 (TTTAAA) site in the A allele.



Alexander Dobrovic, Ph.D.
Chief Medical Scientist
Department of Haematology-Oncology
The Queen Elizabeth Hospital
Woodville, SA 5011, Australia

Tel 011 (or your countries' international code) 61-8-8222 7676
Fax 011 (or your countries' international code) 61-8-8222 6046
adobrovi at medicine.adelaide.edu.au





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