needed: quick & dirty plant DNA extraction for PCR

tarantulito tarantulito at
Mon Feb 15 13:11:37 EST 1999

i am attempting an experiment to cut time and expense in processing
large numbers of small-diameter plant stems for subsequent PCR
detection of MLOs. i cannot find the original biotechniques article 
i intended to use, which described a brief grinding of tissue, quick
boil prep, and immediate PCR of dilutions taken directly from the
boiling prep. can someone direct me to this or a similar protocol?

john rascoe
department of entomology
and plant pathology
110 noble research center, OSU
stillwater, ok  74078

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