Proteinase K to get rid of RNase?

PSarfati psarfati at aol.com
Mon Feb 15 10:42:47 EST 1999


>Does anyone know if it is possible to digest DNA primer for RT using
>proteinase K in order to get rid of RNase? Proteinase K can then be
>taken away by extraction using penol/chloroform?  I have checked the
>proteinase K is RNase free by incubation RNA with it for 2 hours @37

We usualy directly use our RT primer without any specific step for RNAse
destruction.  In fact, dilution of your crude sysnthesis is so big that if any
RNase is present, its amount in the RT tube will be extremly low.




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