X-Gal assay

[Vladimir Rotrekl]

You can use p-nitrophenolglucopyranosid and then you need only
spectrophotometer (412nm).
It is nice, becouse the reaction product formation is visible by eye.

Good luck

Vladimir Rotrekl
Dept of Biochemistry
Masaryk University
61134 Brno
Czech Republic


Chi-Kuang Wen wrote in message <36C39C8B.2E41664B at wam.umd.edu>...
>Dear Netters:
>
>Can someone give me a reliable protocol to test beta-galactosidase
>activity?  I have a protocol but it is hard to get consistant result.
>Do I need to do enzyme kinetics to obtain its activity?
>
>Thanks in advance.
>
>--
>cwen at wam.umd.edu
>http://www.wam.umd.edu/~cwen
>
>Chi-Kuang Wen, Research Associate
>Cell Biology and Molecular Genetics
>University of Maryland
>College Park, MD 20742
>USA
>
>
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