Tween 20 in Western blots

[Annette C. Hollmann]

In article <7aes3c$oc$1 at carroll.library.ucla.edu> "Frank Pajonk" <fpajonk at ucla.edu> writes:
>Hi Netters
>
>does anybody have experience with 5-fold too high concentrations of Tween 20
>(0.25%) in wash- and incubation buffers for western blots? Does it inhibit
>protein binding or HRP-activity?
>

I routinely use 0.2% Tween with good results, so 0.25% should not
interfere with protein binding. I'm not sure about HRP activity as I
always do the final sets of washes in TBS with no Tween.

Annette