Tween 20 in Western blots

Frank Pajonk fpajonk at
Wed Feb 17 11:54:32 EST 1999

Hi Netters

does anybody have experience with 5-fold too high concentrations of Tween 20
(0.25%) in wash- and incubation buffers for western blots? Does it inhibit
protein binding or HRP-activity?


Dr. Frank Pajonk
Dpt.of Radiation Oncology
Experimental Division
UCLA School of Medicine

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