kanamycin resistance anomoly : cultures vs. plates

Rafael Maldonado rafamaldo at geocities.com
Wed Feb 17 15:16:11 EST 1999


kresten at my-dejanews.com wrote:
> 
> This is just an idea.
> 
> Could the problem have to do with phage infection eg. Tn5?

Hum, Tn5 is not a phage... it is a transposon. Sure, it gives resistance
to Km, but it should not be present in a wt E. coli culture, and it
wouldn't explain the go and fro of the resistance.

-Rafa

-- 
Rafael Maldonado
Division of Genetics
University of Alicante, Spain
rmaldonado at ua.es



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