kanamycin resistance anomoly : cultures vs. plates

[Rafael Maldonado]

kresten at my-dejanews.com wrote:
> 
> This is just an idea.
> 
> Could the problem have to do with phage infection eg. Tn5?

Hum, Tn5 is not a phage... it is a transposon. Sure, it gives resistance
to Km, but it should not be present in a wt E. coli culture, and it
wouldn't explain the go and fro of the resistance.

-Rafa

-- 
Rafael Maldonado
Division of Genetics
University of Alicante, Spain
rmaldonado at ua.es