Why low DNA yields from fungi

areti at total.net areti at total.net
Thu Feb 18 10:37:54 EST 1999


On Sun, 14 Feb 1999 20:41:45 GMT, milana100 at nospam.hotmail.com (Hans)
wrote:

>When isolating DNA from fungi we have repeatedly observed extremely
>low DNA yields that are not even detectable on agarose gels or with
>OD260 measurements. We have tried several methods of DNA isolation
>such as phenol/chloroform and spin column techniques.
>Is there an explanation for this?
>
>
>Hans
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How do you break the cells? You really have to use glass beads (I use
sand grains) to efficiently break the cell wall. 



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