Primers incorporating restriction sites

Zophonias O. Jonsson zjons at
Wed Feb 17 06:14:48 EST 1999

In article <Pine.SOL.3.96.990217093819.22136A-100000 at bute>, Jonathan
Howarth <jrh2 at> wrote:

> Can anyone tell me how well SacI and BamHI cut when 3 nucleotides from the
> end of a PCR fragment. These sites were incorporated into the 5' ends of
> the primers (+2 additional nucleotides ie. 5'NNGGATCC........3'). 

BamHI, yes definitely,  SacI, yes but can't guarantee the efficiency.

> I remember once seeing a table of such information for all the most common
> enzymes. Could anyone also tell me where I can find this table.

New England BioLabs catalog 1998/99 page 258
Zophonias O. Jonsson
Institut fur Veterinarbiochemie               Tel: (41-1)-635-54-76
Universitat Zurich-Irchel                     Fax: (41-1)-635-68-40
Winterthurerstrasse 190
CH-8057 Zurich
Switzerland                            <>

More information about the Methods mailing list