Ligation problem

Andrew Leung leungkc at
Thu Feb 18 09:53:36 EST 1999

Hi all,

    Specifically, I cut both my pcr product and my favorite vector with two
restriction enzymes. Then, I perform ligation reaction which is supposed to
give transformant of having insert in right orientation. Now, I can't get
any transformant. Since all control experiments were OK, I guess the problem
resides on ligation parameters.

    Is there any suggestion in improving my ligation efficiency?

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