Glutathione and ion-exchange

[kresten at my-dejanews.com]

Does anybody have any experience with running glutathione (both GSH and
GSSG)on an ion-exchange column.

More specifically I would like GSH and GSSG *not* to bind to an SO3- based
cation-exchange column. I do not want any salt present. I am also going to
bind a protein to the column at the same time, so I want to use a low pH (eg.
3), where the protein will bind strongly but the glutathione not.

Thank you
Kresten



The address kresten at my-dejanews.com is for spambots only. Please mail me
at LysLeuLeu at crc.dk , transforming the pre at -part into my initials.

Kresten Lindorff Larsen
Dept. Yeast Genetics
Carlsberg Laboratory, Denmark

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