Glutathione and ion-exchange

kresten at kresten at
Fri Feb 19 06:39:38 EST 1999

Does anybody have any experience with running glutathione (both GSH and
GSSG)on an ion-exchange column.

More specifically I would like GSH and GSSG *not* to bind to an SO3- based
cation-exchange column. I do not want any salt present. I am also going to
bind a protein to the column at the same time, so I want to use a low pH (eg.
3), where the protein will bind strongly but the glutathione not.

Thank you

The address kresten at is for spambots only. Please mail me
at LysLeuLeu at , transforming the pre at -part into my initials.

Kresten Lindorff Larsen
Dept. Yeast Genetics
Carlsberg Laboratory, Denmark

-----------== Posted via Deja News, The Discussion Network ==----------       Search, Read, Discuss, or Start Your Own    

More information about the Methods mailing list