Removal 3' overhangs!!! Help

Valeri Krougliak krougv01 at doc.mssm.edu
Fri Feb 19 16:38:32 EST 1999


I usually use Klenow enzyme. Add .5u of Klenow to your reaction mixture,
incubate 5 min. at Rt without dNTPs, then add dNTPs to a final concentration
of .2mM and incubate at Rt for another 20 min. Work excelent for me.

amherrera at CIGB.EDU.CU wrote in message ...
>    Dear netters,
>
>    Could Any One of You offer me a protocol of removal 3'
>    overhangs using T4 DNA Polimerase?
>
>    Kind Regards
>    Diogenes QV





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