anti-activated erk problems

Andrew Schrader andrews at pharm.usyd.edu.au
Sun Feb 21 19:49:46 EST 1999


A colleague of mine has been using anti-activated erk
(polyclonal/rabbit; Promega) in western blots and has recently found
problems in detecting the protein (since receiving a new batch!).

A new batch is on its way, but it would be interesting to hear from
those of you who have experienced similar problems with this system.

Suggestions for alternative sources would also be appreciated -
especially those companies with suppliers in Australia.

The secondary antibody (against rabbit IgE) is fine as it has been
tested on a small amount of the old batch that was remaining.

--
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Dr Andrew P. Schrader
Postdoctoral Fellow
(Respiratory Research Group)
Department of Pharmacy (A15)
The University of Sydney NSW 2006

Telephone:
   Office (Rm S222):         +61 2 9351 6099
   Laboratory (Rm N334):  +61 2 9351 2670/3848
   Mobile/Voice Mail:        +61 4 1169 1961
Facsimile (business):       +61 2 9351 4391
Email:                                andrews at pharm.usyd.edu.au
URL: http://www.usyd.edu.au/su/pharmacology/resprg.html
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