pCMV-TAG vectors

monica narang m.narang at cmcb.uq.edu.au
Mon Feb 22 02:04:16 EST 1999


Has anyone used Stratagene's pCMV vector called pCMV-Tag1? It has two TAGs
within it (c-myc and FLAG) allowing one to use either TAG in-frame with a
cDNA of your desire (or so the company claims). I want to use the TAGs as
targets for immunoprecipitations and by using a TAGGED vector, I am hoping
to reduce the amount of cloning that I am doing presently.

Can anyone offer any advice on whether this vector is effective, or if
these TAGs work in IPs?



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