Making deletions with PCR

Jeremy Murray bmbjmm at bmb.leeds.ac.uk
Mon Feb 22 19:28:24 EST 1999


Hi Peter
Check out references to megaprimer PCR
esp. the work of Saker & Somer
also check the web
there are some good sites around
can't remember the URLs off the top of my head
but a very good one in pennsylvania
use a search engine
have fun!
j

Peter Russell wrote:

> How long of a deletion is it possible to make using PCR?  I have
> a need to remove about 140 bp and PCR would be a convenient method
> if it will work.
>
> Peter Russell
> Reed College
> yeast at reed.edu

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Jeremy Murray                            MAIL: bmbjmm at bmb.leeds.ac.uk
Biochemistry & Molecular Biology         TEL:  +44 113 233 2591
Leeds University, LEEDS LS2 9JT          FAX:  +44 113 233 3167
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