The protocols to extract genomic DNA from whole blood
lacyp at unicorn.it.wsu.edu
Mon Feb 22 14:07:55 EST 1999
You may want to repeat the RBC lysis step to get a cleaner prep. I had
some Elk samples that I used with the Puregene kit and this helped quit a
bit. You might also include Prot. K with the Cell lysis buffer step.
Re: the DNAzol kit... what's the maximum number of cells it will handle?
You might be overloading the system.
Paul Rasmussen (paul at ib3.gmu.edu) wrote:
: Gentra Systems' DNA Puregene kit works well. Very easy and good yields.
: Gunnaporn Veerachato wrote:
: > Dear all
: > I would like to extract genomic DNA from elephant whole blood. I
:> have tried DNAZol Reagent but the extracted genomic DNA was in brown
:> color and it does not solubilize well, although I have tried 8mM NaOH
:> to solubilize it according to the protocol. Does anyone have other
:> better protocols to extract genomic DNA from the whole blood?
: > Thank you in advance
: > Sincerely Yours,
: > Gunnaporn Veerachato
: > vgunnapo at mailcity.com
: > Get your FREE Email at http://mailcity.lycos.com
: > Get your PERSONALIZED START PAGE at http://personal.lycos.com
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