What polymerase did not add extra A at 3'end

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Tue Feb 23 04:33:24 EST 1999


In article <7as3no$a35$1 at panix3.panix.com>, Ian A. York
<iayork at panix.com> writes
>Even if you use a Taq pol, some of the products are blunt.

Especially if you don't use a 5-10min soak at 72C after the final cycle.

Duncan
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk



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