What polymerase did not add extra A at 3'end
Dr. Duncan Clark
Duncan at nospam.demon.co.uk
Tue Feb 23 04:33:24 EST 1999
In article <7as3no$a35$1 at panix3.panix.com>, Ian A. York
<iayork at panix.com> writes
>Even if you use a Taq pol, some of the products are blunt.
Especially if you don't use a 5-10min soak at 72C after the final cycle.
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....
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