What polymerase did not add extra A at 3'end

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Tue Feb 23 04:33:24 EST 1999

In article <7as3no$a35$1 at panix3.panix.com>, Ian A. York
<iayork at panix.com> writes
>Even if you use a Taq pol, some of the products are blunt.

Especially if you don't use a 5-10min soak at 72C after the final cycle.

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382

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