competitive RT-PCR - how rare is the mRNA

Andrew G. gosbella at vaxc.cc.monash.edu.au
Tue Feb 23 04:11:52 EST 1999


Hi
I am doing competitive RT-PCR and measuring my target mRNA as copies per ug
total RNA. Can anyone suggest ball-park ranges for rare, moderate and
abundant for mRNA levels in terms of copies per ug total mRNA?

Is there anything in the literature on this?

thanks for your suggestions

Andrew
gosbella at vaxc.cc.monash.edu.au



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