Can one make a TOPO vector?

[Peter Wang]

 Tobias Straub wrote:

> ...but there's still another problem: in topo cloning kits there's vaccinia virus
>
> topo I. this is a special type IB enzyme that has a specific recognition
> sequence CCTT/. other eukryotic enzymes don't have such a sequence specificity.
> ....

Dear Tobias:

Right, to me it seemed the main obstacle to making one's own topo-cloning vectors
is getting the vaccinia virus topoisomerase protein.  I guess no one sells it?  Is
it difficult to express it yourself?  And if you do make your own topo-cloning
kits, will Invitrogen or some other patent lawyer come after you?

Cheers,
- Peter

---------------------------------------------------------
Peter Wang, M.D., Ph.D.
MRC Centre for Protein Engineering,
Hills Road, Cambridge, CB2 2QH, England

Tel (01223) 402104  (international calls +44-1223-402104)
Fax (01223) 402140  (     "          "   +44-1223-402140)

CrossTalk Society: http://www.corpus.cam.ac.uk/Activities/pcts/
---------------------------------------------------------