Stratagene's Quick Change Kit..Wow!

John R. McQuiston zje8 at cdc.gov
Fri Feb 26 09:10:09 EST 1999


Hi Everyone,

I just used the Quick Change SDM Kit for the first time and for a large 
mutation 6bp del. 2bp change.  I was in awe.  I screened 17 clones by 
sequencing and EVERY single clone had the correct mutation.  I was hoping for 1 
or 2.  I would highly reccommend this procedure since it's as easy as setting 
up a PCR reaction , a restriction digest and transformation.  

I'm impressed, and also not affiliated with Stratagene in any way.

John R. McQuiston
Centers for Disease Control.





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