Stratagene's Quick Change Kit..Wow!
John R. McQuiston
zje8 at cdc.gov
Fri Feb 26 09:10:09 EST 1999
Hi Everyone,
I just used the Quick Change SDM Kit for the first time and for a large
mutation 6bp del. 2bp change. I was in awe. I screened 17 clones by
sequencing and EVERY single clone had the correct mutation. I was hoping for 1
or 2. I would highly reccommend this procedure since it's as easy as setting
up a PCR reaction , a restriction digest and transformation.
I'm impressed, and also not affiliated with Stratagene in any way.
John R. McQuiston
Centers for Disease Control.
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