What is defined LB??

Byoung Heon Kang heony at postech.ac.kr
Fri Feb 26 01:27:31 EST 1999


I use IPTG inducible expression system.
But, I have some problem in expressing my protein in E.coli.
Then, one of my instruction manual suggest to use 'defined LB' media.
(for the purpose of eliminating lactose from the broth.)
But I have not heard about 'defined LB'
In maniatis's 'molecular cloning', a few mininal media is listed, I guess...
But I don't know what is the best one....

If you have any fomulas meet for this purpose...
help me...

thanks






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