cleavable C-tern His tag

Mark Bowen mb at
Fri Jan 8 10:15:58 EST 1999

I am trying to find an expression vector with a C-terminal, cleavable
His tag for protein expression.  Neither Novagen nor Qiagen sells such a
vector.  Is there one available anywhere or do I have to make it
myself?  I realize that this will leave additional residues upon
cleavage, but the N terminus needs to be unaltered as it is involved in
protein:protein interactions.

Mark Bowen
HHMI/Yale University
Dept. of Molecular Biophysics and Biochemistry
mb at

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