cDNA array

Narendra Kaushik n.kaushik at ic.ac.uk
Mon Jan 11 09:38:07 EST 1999


I think that your problem is contaimination during RT reaction. Have you
checked your
counts after purification? How much RNA are you using? How are you
purifying?
I get very good results by using G-25 spin coulmns (Pharmacia). I
suggest to check:
1- Integrity of your RNA.
2- Your solutions.
3- Your method of purification.

I hope that helps.

Narendra Kaushik
Neuromuscular Diseases,
ICM London W6 8RP
n.kaushik at ic.ac.uk





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