spinning bugs (was re: pGEX Protein EXpression HELP with Method)
Richard P. Grant
r_grant at see.sig.for.address
Mon Jan 18 03:18:48 EST 1999
In article <369EC503.5080 at REMOVETHISmolsci.csiro.au>,
David.Tucker at REMOVETHISmolsci.csiro.au wrote:
>Richard P. Grant wrote:
>> In article <E100mG6-0000le-00 at mailhost.rpms.ac.uk>, mjones at rpms.ac.uk ("Dr
>> Mick Jones") wrote:
>> >Transfer 1.5 ml of the cultures to microfuge tubes, spin for 3 minutes and
>> Far too long. 1 minute max at 13k x g.
>ligations recently and was wondering - in light of the above - whether
>perhaps this spin was too harsh on the bugs?
heh, I only think 3 minutes is too long because it's (a) three times
longer than really necessary, and (b) makes the bugs more difficult to
resuspend. Doon't know anything about any damage to them :)
Richard P. Grant MA DPhil | rgrant at cmtech.co.uk
Senior R&D Scientist | work: www.cmtech.co.uk
Cambridge Molecular | home: www.avnet.co.uk/adastra
When MS created NT, how did they get an infinite number of monkeys?
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