pH and FISH

Ian Roberts ir210 at
Thu Jan 21 06:14:29 EST 1999

Dear All,

Does anyone know if the pH of a DNA probe interferes with its hybridisation?
 I ethanol ppt my probes before resuspending in the Hyb Buffer.  As this
involves 3M NaOac at pH 5.2, I wondered if residual salt left by inadequate
70% ethanol washes could upset the subsequent hybs?

I would appreciate any thoughts or comments.



Ian Roberts
Pathology Department
Cambridge University
Tennis Court Road
Cambridge.  CB2 1QP

Tel +44 (0)1223 333735
Fax +44 (0)1223 333730

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