pH and FISH
ir210 at mole.bio.cam.ac.uk
Thu Jan 21 06:14:29 EST 1999
Does anyone know if the pH of a DNA probe interferes with its hybridisation?
I ethanol ppt my probes before resuspending in the Hyb Buffer. As this
involves 3M NaOac at pH 5.2, I wondered if residual salt left by inadequate
70% ethanol washes could upset the subsequent hybs?
I would appreciate any thoughts or comments.
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