Thermostable DNA helicase Wonder anybody has ever used DNA helicase or topoisomerase in ABI fluoresent sequencing with "difficult" templates such as those with strong secondary structures. Does anybody know if there is any commercial DNA helicase available? Any advice is greatly appreciated.

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Fri Jan 22 05:01:18 EST 1999


In article <36A7E1C1.3D65 at indiana.edu>, Joe Smith <jsmith at indiana.edu>
writes
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>Dear Netters
>
>Wonder anybody has experience using DNA helicase or topoisomerase in ABI
>fluoresent sequencing with "difficult" templates such as those with
>strong secondary structures. Does anybody know if there is any
>commercial DNA helicase available? Any advice is greatly appreciated.
>
>Joe

In a word, Yes! Look out for Fidelase. It is a hyperthermophilic
topoisomerase V from Methanopyrus kandleri ( and already under US
patent) sold for sequencing. A web search should find it.

Duncan

-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk



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