WESTERN BLOTTING OF 15KDa PRotein

Tom Vink tvink at xs4all.nl
Wed Jul 7 02:44:43 EST 1999


On 6 Jul 1999 10:17:11 -0700, fesquet at crbm.cnrs-mop.fr (Didier
Fesquet) wrote:

>
>>hi,
>>
>>working with a 15KDa protein , we experience difficulties detecting this
>>protein by WB. indeed, we believed that the protein is going throught the
>>nitrocellulose membrane even 0.1micron! . does anybody know how we can
>>sort this out!
>>
>>any help wellcome
>>
>>didier
>>
>>
>
>*******************************************************************
>Dr Didier FESQUET
>
>Centre de  Recherche de Biochimie Macromoleculaire
>CNRS UPR 1086
>1919, route de mende
>34293 Montpellier cedex 5 (FRANCE)
>Tel: (33) (0)4-67-61-33-79 ou 33-72
>FAX: (33) (0)4-67-52-15-59
>From abroad do not dial (0)
>email: fesquet at crbm.cnrs-mop.fr
>
>*************************************************
>
Use PVDF instead of nitriocellulose



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