Leucocytes and Falcon tubes
M. Johan Broekman
hanbroekman at csi.com
Thu Jul 22 20:56:29 EST 1999
"Richard P. Grant" wrote:
> Does anyone know if white cells in whole blood stick to 50 ml Falcon tubes
> to any significant extent?
> This obviously affects the type of vessel we use for storage and collection.
> Richard P. Grant MA DPhil | rgrant at cmtech.co.uk
> work: www.cmtech.co.uk | home: www.avnet.co.uk/adastra
> -- 'Bring me the hedge of Alfredo Garcia' --
It's likely that leukocytes would stick to some kinds of plastic, especially
when (minimally) activated. Of course the monocytes/macrophages would do so
most. What kind of plastic are you using? Polypropylene may be least sticky.
What anticoagulant are you using? Heparin is very often used, but may not be
the ideal choice. ACD or CPD may preserve the cells (ATP content) better.
Also, the method of collection could affect the results. Free flow, rather than
suction by syringe would be best, discarding the first few ml of blood which may
contain "tissue juice" from the stick. Although it may look sacrificial, a 17
gauge needle doesn't hurt any more than a 21 gauge one if handled properly (I
have most often been on the receiving end).
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