Question on DEPC

Jeremy Murray bmbjmm at bmb.leeds.ac.uk
Sat Jul 31 15:37:34 EST 1999


check this article out

http://www.abrf.org/ABRFNews/1992/December1992/dec92oligo.html

hth, jez

luc wrote:

> Protocols for RNase free solutions state that DEPC should be left for at
> least one hour at 37 °C folowed by inactivation through autoclaving.
> I was tought that DEPC in water is decomposing into ethanol and CO2 in a
> matter of minutes. So what is the point of incubating it that long?
> ( i usually shake it for a few minutes and then autoclave. it gets RNAse
> free as well.)
>
> --
> Luc  Nelles
> VIB 07 department for cell growth, differentiation and development
> University of Leuven, Herestraat 49
> B-3000 Leuven, Belgium
> Email:  luc at sgi.celgen.kuleuven.ac.be
> Tel. 32-16-345929
> Fax. 32-16-345933




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