DNA extraction from recalcitrant tissues

[Gonzalo]

In article <3752834D.5AC99F11 at sgen.slu.se>, Mathieu.Ingouff at sgen.slu.se
(Mathieu Ingouff) wrote:

 > Ratio: A260/A280: 1.6-1.7
 >            A260/A230: >2 (i assume it is free of polysaccharides)
 > 
 > I can get nice bands by PCR but i can't cut 10 ug even after a week
 > using 5 shots of enzymes (90U each time)

Are you sure that it is not cleaved? I use a similar protocol with
olive tree and pine and they are easily fragmented.

Gonzalo


-- 
Manuel G. CLAROS, Ph.D.                             claros at uma.es
Dpt Biologia Molecular y Bioquimica              (remove ANTISPAM
Universidad de Malaga, 29071 Malaga (Spain)        when replying)
http://www.nitrogeno.uma.es/FMP/