wrong orientation of ligated fragment

Gregor Kopitar gregor.kopitar at ijs.si
Tue Jun 1 08:56:33 EST 1999

I am trying to ligate  1500bp fragment into 6200bp expression vector at Bgl
ll restriction site. All of the positive clones have the fragment in wrong
orientation and I do not have anymore ideas, what to do.
I welcome any suggestion.

Ursa Pecar, PhD Student
ursa.pecar at ijs.si

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