synthetic E coli promoter?

John Dixon jpcd100 at mole.bio.cam.ac.uk
Tue Jun 1 18:23:11 EST 1999


Hi all, 

I wish to place an oligo upstream of the neomycin phosphotransferase
cassette in a construct of mine to so that it also confers kanamycin
resistance in E coli. I have a convenient site in between the
eukaryotic promoter and the neo ATG. 

What is the mininmum consensus sequence for a promoter active in E coli?

Thanks for any help

JD

jpcd100 at mole.bio.cam.ac.uk



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