synthetic E coli promoter?

John Dixon jpcd100 at
Tue Jun 1 18:23:11 EST 1999

Hi all, 

I wish to place an oligo upstream of the neomycin phosphotransferase
cassette in a construct of mine to so that it also confers kanamycin
resistance in E coli. I have a convenient site in between the
eukaryotic promoter and the neo ATG. 

What is the mininmum consensus sequence for a promoter active in E coli?

Thanks for any help


jpcd100 at

More information about the Methods mailing list