desalting DNA

C.S. Wilding BGYCSW at leeds.ac.uk
Wed Jun 2 02:16:44 EST 1999


In article <199906011406.QAA21284 at intwww.zit.med.uni-tuebingen.de>,
   Wolfgang.Schechinger at med.uni-tuebingen.de ("Wolfgang Schechinger") 
wrote:
>Hi Craig, 
>
>What actually is your application?

Hi,
It is genomic DNA isolated from snails. It digests with MspI but not EcoRI, 
and I am led to believe that (from Kramel Biotech catalogue: supplier) 
EcoRI is very salt sensitive, so am making the assumption that I have salt 
left. The DNA is isolated with a CTAB method that includes a very high 
concentration of NaCl so I suppose this is a reasonable assumption.
Thanks for all the suggestions so far!
Craig.



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