Phage contaminatio

Marcus Wernitz viacx at mlucom6.urz.uni-halle.de
Wed Jun 2 02:58:03 EST 1999


I have got a problem with my cosmid containing Ecoli.
All clones seem to be cataminated with phages. In my overnight cultures
all the cells seem to be lysed.
When I try to make a plasmid prep I only get smallest amount of DNA.
To get rid of the phages I streaked the cells on a new LBamp plate to
get single colonies, but they still lyse in ON-cultures.
Has anybody got a similar proble and got a solution to it?

Thank you in advance,

Marcus Wernitz

Diplombiologe
Marcus Wernitz
Martin-Luther-Universitaet Halle-Wittenberg
Landwirtschaftliche Fakultaet
Institut fuer Pflanzenzuechtung und Pflanzenschutz
Lehrstuhl fuer Phytopathologie und Pflanzenschutz
Ludwig-Wucherer-Str. 2
06108 Halle a.d.Saale
Germany

Tel.: +49 (0)345-5522666 Fax : +49 (0)345-5527120
E-Mail: Wernitz at Landw.Uni-Halle.de

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Paracelsusstr.6
06114 Halle a.d.Saale
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http://Wernitzm.home.pages.de
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