Phage contaminatio

[Marcus Wernitz]

I have got a problem with my cosmid containing Ecoli.
All clones seem to be cataminated with phages. In my overnight cultures
all the cells seem to be lysed.
When I try to make a plasmid prep I only get smallest amount of DNA.
To get rid of the phages I streaked the cells on a new LBamp plate to
get single colonies, but they still lyse in ON-cultures.
Has anybody got a similar proble and got a solution to it?

Thank you in advance,

Marcus Wernitz

Diplombiologe
Marcus Wernitz
Martin-Luther-Universitaet Halle-Wittenberg
Landwirtschaftliche Fakultaet
Institut fuer Pflanzenzuechtung und Pflanzenschutz
Lehrstuhl fuer Phytopathologie und Pflanzenschutz
Ludwig-Wucherer-Str. 2
06108 Halle a.d.Saale
Germany

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