synthetic E coli promoter?
Dr. Duncan Clark
Duncan at nospam.demon.co.uk
Wed Jun 2 04:01:53 EST 1999
In article <020619990023114063%jpcd100 at mole.bio.cam.ac.uk>, John Dixon
<jpcd100 at mole.bio.cam.ac.uk> writes
>What is the mininmum consensus sequence for a promoter active in E coli?
Not sure about the minimum but don't forget an rbs. By the time you have
-35, -10 and the rbs plus say 5 bases to an ATG you must be looking at
40 plus bases. Years ago I'm sure Pharmacia used to sell a promoter
linker. If you can find an old catalogue that may help.
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....
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