synthetic E coli promoter?

Dr. Duncan Clark Duncan at
Wed Jun 2 04:01:53 EST 1999

In article <020619990023114063%jpcd100 at>, John Dixon
<jpcd100 at> writes
>What is the mininmum consensus sequence for a promoter active in E coli?

Not sure about the minimum but don't forget an rbs. By the time you have
-35, -10 and the rbs plus say 5 bases to an ATG you must be looking at
40 plus bases. Years ago I'm sure Pharmacia used to sell a promoter
linker. If you can find an old catalogue that may help.

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382

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