desalting DNA

Dr. Duncan Clark Duncan at
Wed Jun 2 04:06:22 EST 1999

In article <7j2lod$89g_001 at>, C.S. Wilding
<BGYCSW at> writes
>It digests with MspI but not EcoRI, 
>and I am led to believe that (from Kramel Biotech catalogue: supplier) 
>EcoRI is very salt sensitive, so am making the assumption that I have salt 

It is salt sensitive in that it likes salt in it's assay buffer i.e it
still works in 150mM NaCl! Don't ever use EcoR I is a low salt assay
buffer as you will get star activity.

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382

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