desalting DNA

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Wed Jun 2 04:06:22 EST 1999


In article <7j2lod$89g_001 at leeds.ac.uk>, C.S. Wilding
<BGYCSW at leeds.ac.uk> writes
>It digests with MspI but not EcoRI, 
>and I am led to believe that (from Kramel Biotech catalogue: supplier) 
>EcoRI is very salt sensitive, so am making the assumption that I have salt 
>left. 

It is salt sensitive in that it likes salt in it's assay buffer i.e it
still works in 150mM NaCl! Don't ever use EcoR I is a low salt assay
buffer as you will get star activity.

Duncan
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk



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