desalting DNA

Guy Hoelzer hoelzer at
Wed Jun 2 11:55:38 EST 1999

In article <199906011610.KAA23666 at nestor.NMSU.Edu>, hroychow at NMSU.EDU
("Hiranya S. Roychowdhury") wrote:

> So, if somebody wants DNA with absolutely no salt , s/he may soak the DNA
> pellet in 70% EtOH for extended periods and do more than just one wash. This
> is what I do when I want to get rid of salts or pigments: After the DNA is
> pelleted/spooled out from the precipitaion, I let the pellet/spooled DNA sit
> in 70%EtOH for 15 min before spinning/spooling out the DNA. I repeat this
> two/three times. 

Will this method get rid of excess amounts of EDTA, as well as salts like NaCl?

Guy Hoelzer
Department of Biology
University of Nevada Reno
Reno, NV  89557

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