2-mercaptoethanol and DTT

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Thu Jun 3 10:18:30 EST 1999

In article <37567F17.6785C194 at yahoo.com>, Mismatch 420
<mismtch420 at yahoo.com> writes
>Anyone care to comment on the interchangeability(sp?) of these 2

No real problem. 10mM B-mercaptoethanol for 1mM DTT. I believe that B-
mercaptoethanol goes off faster but is a lot cheaper than DTT for say
medium scale protein purification i.e. buffer on 20 litre scale etc.

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382

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