2-mercaptoethanol and DTT

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Thu Jun 3 10:18:30 EST 1999


In article <37567F17.6785C194 at yahoo.com>, Mismatch 420
<mismtch420 at yahoo.com> writes
>Anyone care to comment on the interchangeability(sp?) of these 2
>compounds?

No real problem. 10mM B-mercaptoethanol for 1mM DTT. I believe that B-
mercaptoethanol goes off faster but is a lot cheaper than DTT for say
medium scale protein purification i.e. buffer on 20 litre scale etc.

Duncan 
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk



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